This monograph for sodium dodecyl sulfate provides, in addition to common physical constants, a general description. Size of sodium dodecyl sulfate micelles in aqueous. Sodium lauryl sulfate is a mixture of sodium alkyl sulfates consisting chiefly of sodium lauryl sulfate ch 3 ch 2 10 ch 2 oso 3 na. A twice recrystallized quality of sds with excellent qualities for denaturing proteins before gel electrophoresis, molecular weight sieving and many other applicationsharewood k. Sds represents a potentially effective topical microbicide, which can also inhibit and possibly prevent infection by various enveloped and nonenveloped viruses such as. It is a component of many cell lysis buffer formulations, including ripa buffer. If you continue and accept all cookies, you will receive all of the cookies that we use on the site.
The binding of sodium dodecyl sulphate to proteins by equilibrium dialysis was investigated. Sodium lauryl sulfate sulfuric acid monododecyl ester sodium salt. Sodium dodecyl sulfate sds, white powder, electrophoresis. First aid measures general advice consult a physician. It consists of a 12carbon tail attached to a sulfate group, that is, it is the sodium salt of dodecyl. Dodecyl sodium sulfate recommended use laboratory chemicals. Choose from over 850 chemical products in chemical grades, sizes and concentrations to meet your needs. Effect of light on selfassembly of aqueous mixtures of.
Sds certificate of analysis product specification technical inquiry stock no. High purity, submicron and nanopowder forms may be considered. Resources msds material safety data sheets msds sodium dodecyl sulfate, 10%. We have recently shown that this protein is a cytoskeletal component immunologically related to spectrin and that it is directly. It is mainly used in detergents for laundry with many cleaning applications.
Sodium dodecyl sulfate sds is an alkyl sulfate, anionic detergent used frequently in protein electrophoresis and protein solubilization methods. Effects of butanol during alkali treatment and citrate in post alkali treatment were monitored in terms of morphology and the chemical properties of microcapsules. Sodium dodecyl sulfate other means of identification product no 1894, 4095, l050. Sodium lauryl sulphate material safety data sheet cas no.
Sodium dodecyl sulfate sdspolyacrylamide gel electrophoresis of the purified channel protein extract is shown in fig. Product and company identification product name dodecyl sulfate sodium salt cat no. Physical and chemical properties molecular formula nac12h25oso3. Sodium dodecyl sulfate sds 10% wv stock solution contains 10% sodium dodecyl sulfate. Sigmaaldrich material safety data sheet date printed. Sodium lauryl sulfate 1288 mgkg rat 580 mgkg rabbit 3900 mgm3 rat 1 h sodium sulfate 0 mgkg rat not listed not listed sodium chloride 3 gkg rat 10 gkg rabbit 42 gm3 rat 1 h irritation irritating to eyes, respiratory system and skin toxicologically synergistic products no information available.
This allows for relatively linear separation in electrophoresis, most often page polyacrylamide gel electrophoresis, sodium lauryl sulfate. Sodium dodecyl sulfate 10% wv stock solution safety data sheet supersedes revision. Most of the proteins studied bound 90100% of their weight of sodium dodecyl sulphate. Dodecyl sodium sulfate, dodecyl sulfate sodium salt, lauryl sulfate sodium salt, sds, sodium lauryl sulfate. Sodium dodecyl sulfate dodecyl sodium sulfate lauryl sodium sulfate. In a fourcomponent analysis of the data, it appears that the lifetime o3 of the positronium triplet state ops in the aqueous phase decreases. Concentration sodium dodecyl sulphate 1512 2057881 4. Health effect of inhaling sds sodium dodecyl sulphate category. I want to measure the size of the precipitates using malvern mastersizer, which needs accurate refractive. Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of the safety data sheet emergency telephone number. Sodium sulfate anhydrous is an electrolyte replenisher and is used in isosmotic solutions.
The gross conformation of proteinsodium dodecyl sulfate. Sodium dodecyl sulphate sds cas number 1512 molecular weight c 12 h 25 o 4 sna 288. Sds solution, 20% sodium dodecyl sulfate solution, molecular. Safety data sheet creation date 24nov2010 revision date 17jan2018 revision number 6 1. Sodium dodecyl sulfate, molecular biology grade sds. Sodium sulcate procedure transfer about 1 g of sodium lauryl sulfate, accurately weighed, in 10 ml of water, add 100 ml of alcohol and heat at a temperature just below the boiling point for 2 hours. Msds contains all the information required by the cpr. It also serves as a research tool in protein biochemistry. Thermo fisher scientific dodecyl sulfate sodium salt revision date 16jun2011. See section 8 of the msds for personal protective equipment. Dodecyl sulfate, sodium salt appears as white to pale yellow paste or liquid with a mild odor.
Sodium dodecyl sulphate solution standardization to 50. The light influences the dynamic surface tension of. Revised on 03192015 page 1 of 6 safety data sheet sodium dodecyl sulfate 20% 1. This binding ratio on a gram to gram basis is identi cal for all proteins investigated. Sodium dodecyl sulfate skin not available eyes not available respiratory not available ingestion not available carcinogenicity iarc no components of this product present at levels greater than or equal to 0. Sodium dodecyl sulfate 10% wv stock solution safety data. Hazardous thermal decomposition products specific hazards arising from the chemical. I want to measure the size of the precipitates using. Sodium dodecyl sulfate, molecular biology grade sds, is a detergent that is known to denature proteins. Sodium dodecyl sulfate an overview sciencedirect topics. Sodium lauryl sulfate, in science referred to as sodium dodecyl sulfate sds or duponol, is commonly used in preparing proteins for electrophoresis in the sdspage technique. Sodium dodecyl sulfate 20% columbus chemical industries. The micelles formed by sodium dodecyl sulfate sds are studied in aqueous solutions at 294 k as a function of nominal sds concentration c using positron lifetime spectroscopy.
No action shall be taken involving any personal risk or without suitable training. Dodecyl sulfate is incompatible with strong oxidizers. The hazard classification and labelling section shows the hazards of a substance based on the standardised system of statements and pictograms established under the clp classification labelling and packaging regulation. Show this safety data sheet to the doctor in attendance. It is an anionic surfactant used in many cleaning and hygiene products. The interaction of sodium dodecyl sulfate with a wide variety of proteins is characterized by a high binding ratio when the monomer concentration of amphiphile exceeds 5 x 10w4 m. Sds is used to denature proteins with a fixed single negative charge per approximately 2 amino acids. Sodium lauryl sulfate dodecyl sodium sulfate dodecyl sulfate sodium salt sds formula. The substance identity section is calculated from substance identification information from all echa databases. Sodium sulfate may persist indefinitely in the environment, but is not likely to show bioaccumulation or food chain contamination effects.
This laurylgrade sodium dodecyl sulfate sds is a popular anionic detergent for routine protein electrophoresis and cell lysis methods. Uses advised against not for food, drug, pesticide or biocidal product use. You have selected the maximum number of product attributes 3 to compare. Sodium dodecyl sulfate other means of identification.
Material safety data sheet northwest missouri state. When sodium dodecyl sulfate was dissolved in a salt aqueous solution, precipitates formed. Sds solution 10% safety data sheet university of reading. Sodium dodecyl sulfate or sodium dodecylsulfate is generally immediately available in most volumes. Typical sds or lauryl sulfate preparations are mixtures of several different chain lengths c10 to c18. Sodium dodecyl sulfate sds, also known as lauryl sulfate, is an ionic detergent that is useful for the rapid disruption of biological membranes. Filter through a glass filter while hot, and wash with 100 ml of boiling alcohol. We use cookies on our website to help provide you with the best online experience possible. Gives off irritating or toxic fumes or gases in a fire. Sodium ion is the principal cation of the extracellular fluid and plays a large part in the therapy of fluid and electrolyte disturbances. Identification of the substancemixture and of the companyundertaking 1. Thermo fisher scientific dodecyl sulfate sodium salt.
It may be dangerous to the person providing aid to give mouthtomouth resuscitation. We report the use of uv light to control the dynamic surface tensions of mixed surfactant systems containing sodium dodecyl sulfate, sds, and 4,4bistrimethylammoniumhexyloxyazobenzene bromide, btha. Electrophoresis 2 sodium dodecyl sulfate polyacrylamide gel electrophoresis sdspage3 uniform percentage gels 4 scope. Industrial, manufacturing or laboratory use manufacturer. It has a role as a detergent and a protein denaturant.
Material safety data sheet university of maryland, college park. The combined content of sodium chloride and sodium sulfate is not more than 8. American elements produces to many standard grades when applicable, including mil spec military grade. Sodium dodecyl sulphate substance information echa.
Sodium dodecyl sulphate the ncbe university of reading. View or download the sodium dodecyl sulfate msds material safety data sheet or sds for 817034 from merck. It is a key component of many reagents used to purify nucleic acids because of its abilities to quickly disrupt the tissue architecture and to inhibit both rnase and deoxyribonuclease dnase activity. Polyacrylamide gel electrophoresis is used for the qualitative characterisation of 5 proteins in biological preparations, for control of purity and for quantitative determinations. If diluted with water, this chemical released directly or indirectly into the environment is not expected to have a. Sigmaaldrich offers a number of sodium dodecyl sulfate solution products. In the sdshcl and ctabkoh systems, the micellar phase had a fixed. It is also used as fat emulsifier, a wetting agent, a detergent in cosmetics, pharmaceuticals and toothpastes.
Sodium dodecyl sulfate sds, white powder, electrophoresis, fisher bioreagents for protein extraction procedures and sds polyacrylamide gels brand. Sodium sulfate anhydrous is the anhydrous, sodium salt form of sulfuric acid. Warning this product is not for human or veterinary use. It is also incompatible with cationic materials and with acids with ph below 2. Firefighting measures promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. Titrate with 1 n sodium hydroxide volumetric solution, using 0. Mechanism of micelle formation in sodium dodecyl sulfate. As well as the 63kda channel protein, the extract also typically exhibits a 240kda component not essential for channel activity 3. Identification product name sodium lauryl sulfate nffcc cat no. The formulation is a mixture of several different alkyl sulfate chain lengths c10 to c18.
Sodium dodecyl sulfate sds, 10% solution section 5. Sodium dodecyl sulfate sds, c12 thermo fisher scientific. It is used in denaturing polyacrylamide gel electrophoresis for the determination of protein molecular weight. The rigidification of the shell membrane of microcapsules was achieved by alkali treatment in the presence of a small amount of 1butanol to reduce aggregation. Carolina offers the highest quality kits for a handson approach within ap chemistry classrooms. The glycoproteins studied bound 70100% of their weight of sodium dodecyl sulphate, calculated in terms of the polypeptide moiety of the molecule.
Various characterization techniques revealed that the. Identification of the substancemixture and of the companyundertaking product name. Sodium dodecyl sulfate is an organic sodium salt that is the sodium salt of dodecyl hydrogen sulfate. Sodium dodecyl sulfate sds or sodium lauryl sulfate sls, sometimes written sodium laurilsulfate, is a synthetic organic compound with the formula c h 3 ch 2 11 so 4 na. Dissolve the precipitate by washing with 150 ml of water, collecting the washings in a beaker. The substance identifiers displayed in the infocard are the best available substance name, ec number, cas number andor the molecular and structural formulas. The binding of sodium dodecyl sulphate to various proteins. The clp regulation makes sure that the hazards presented by chemicals are clearly communicated to. It is also used in nucleic acid extraction procedures for the disruption of cell walls and dissociation of nucleic acid. Sodium sulfate anhydrous disassociates in water to provide sodium ions and sulfate ions.
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